Studies on Codons for Tyrosyl Transfer Ribonucleic Acid and Lysyl Transfer Ribonucleic Acid of Yeast
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چکیده
منابع مشابه
Chicken liver transfer ribonucleic acid: heterologous interaction of alanine transfer ribonucleic acid with yeast.
We are attempting to determine the nature of specific RNA-protein interactions by study of tRNA charging by homologous and heterologous enzymes. tRNAklatRNARNA3 t were prepared by phenoxyacetylation of alanyl-tRNA and separation on benzoylated DEAE-cellulose (Gillam, Blew, Warrington, von Tigerstrom & Tener, 1968). A partially purified alanyl-tRNA synthetase from yeast forms alanyl-tRNAAla tand...
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Transfer RNA inhibited the tyrosine-dependent ATPpyrophosphate exchange catalyzed by tyrosyl-tRNA synthetase from IIscherichia coli. The inhibition was specific for tRNATY’; it was competitive with tyrosine and noncompetitive with ATP. tRNA modified by periodate oxidation or by limited digestion with phosphodiesterase proved to be a stronger inhibitor of the enzyme than native tRNA; the inhibit...
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Soybean seedlings were examined for the presence of mitochondrial tRNA. Tyrosyl transfer tRNAs from whole cells, from a well characterized mitochondrial preparation, and from a snake venom phosphodiesterase-treated mitochondrial preparation, were compared by reverse phase chromatography. It was concluded that none of the three previously reported tRNA(Tyr) species were mitochondrial. Rather, a ...
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Temperature-sensitive mutations in the isoleucyl-transfer ribonucleic acid (tRNA) synthetase of yeast, ilS(-)1-1 and ilS(-)1-2, were used to examine the role of aminoacyl-tRNA synthetase enzymes in the regulation of ribonucleic acid (RNA) synthesis and enzyme synthesis in a eucaryotic organism. At the permissive temperature, 70 to 100% of the intracellular isoleucyl-tRNA was charged in mutants ...
متن کاملStudies on the fractionation of total transfer ribonucleic acid and purification of an alanine transfer ribonucleic acid from chick embryo.
Chick embryo tRNA, prepared by a simple large-scale method, was fractionated on three different ion-exchange columns. In all cases simple chromatographic patterns for various tRNA species were observed, indicating the presence of only a few major species of tRNA for each amino acid. By repeated chromatography one species of alanine tRNA was purified to approx. 80% purity. T(1) ribonuclease dige...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1968
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1968.tb00397.x